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CRISPR Gene Editing Simulator

Design a guide RNA, watch Cas9 cut DNA at the target site, and choose a repair mechanism to see the outcome.

DNA Double Helix

Step 1: Design Guide RNA

Protein Product

Start by selecting a target sequence...

Guide RNA Design

Click bases to build your 20nt guide sequence (or use preset):

Guide RNA:

Controls

Repair method:

Statistics

0%
On-target
0
Off-target
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Edits Done
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Success Rate
NHEJ: Non-homologous end joining. Fast but error-prone; causes random insertions/deletions (indels).

HDR: Homology-directed repair. Uses a template for precise editing but is less efficient (~10-50% of cells).